Arthrospira platensis ZTS-2024

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan.
• Place of isolation: Lake Balkhash.
• Habitat: saline water ecosystem.
• Isolated by: Zayadan B.K., Salauat D., Toktybay A.K. & Bolatkhan B. at the Laboratory of Biotechnology, al-Farabi Kazakh National University.
• Year of accession to the collection: 2025
• Method of obtainment: isolated from an enrichment culture obtained from a natural sample by successive microbiological plating onto solid mineral medium.
• Genetic features: Wild type.
• Culture condition: Axenic.

Morphology and Structure

Cells belong to the filamentous organization type. Trichomes are multicellular, spirally coiled, non-branching, and occur as individual filaments. Cells are cylindrical, blue-green. The trichome width is usually 5–10 µm, while the trichome length may reach several hundred micrometers depending on the degree of coiling and cultivation conditions. Based on individual cell dimensions, the strain belongs to the group of medium-sized microorganisms (2–20 µm). They do not form colonies; vegetative cells are located within the trichome. Heterocysts and akinetes are absent.

Cultivation

• Nutrient Medium: Zarrouk’s medium (liquid and agarized)
• Temperature: 28–30 °C
• Light Intensity: 50–200 µmol photons/m²/s
• Photoperiod: continuous illumination
• pH: 8.0–9.0.

Physiological Characteristics

Alkaliphile, halophile.

Long-term Preservation Conditions

Maintained on agarized Zarrouk’s medium at 12–14 °C and low light intensity 4–6 µmol photons/m²/s. Maximum storage without subculturing is 2–3 months.

Field of Application

Promising for increasing agricultural crop productivity, bioremediation, and formation of algal-cyanobacterial biofilms.

Bibliography

1. Тоқтыбай А.К., Салауат Д., Болатхан К., Сандыбаева С.К., Ахметова Г.А., Құрметхан Ә.Қ., Санатов С.С., Hrouzek P., Заядан Б.К. Балқаш көлінің тұзды бөлігінің альгофлора құрамын зерттеу және фототрофты микроорганизмдердің аксеникалық дақылдарын бөліп алу // Eurasian Journal of Ecology. – 2026. – Т. 86, № 1. – Б. 80–97. – DOI: 10.26577/EJE86120267.
2. Салауат Д., Сейдалиева Н.М., Асқар С. Микробалдырлар биомассасын алудың биотехнологиялық негіздері және оны қолдану перспективалары. Әл-Фараби атындағы Қазақ Ұлттық университеті MATERIALS International Scientific Conference of Students and Young Scientists «FARABI ALEMI» Almaty, Kazakhstan, April 3-5, 2025.
3. Bolatkhan K., Salauat D., Shalgimbayeva S.M., Akmukhanova N.R., Zayadan B.K., Askar A., Gökoğlu M. Enrichment of Artemia nauplius with Cyanobacterium Arthrospira platensis ZTS-2024 for Feeding Young Russian Sturgeon (Acipenser gueldenstaedtii Brandt, 1833) // ES Food & Agroforestry. – 2026. – DOI: 10.30919/faf1987.