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Qazaqstan Culture Collection of Phototrophic Microorganisms
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QCCPM-CY-066

Synechococcus sp.

Cyanobacteria › Cyanophyceae › Synechococcales › Synechococcaceae › Synechococcus

Description

History of Receipt and Isolation

  • Country of origin: Republic of Kazakhstan.
  • Isolation site: Aktau, industrial zone of the Oil Refinery, production units, point M-13 (at the inlet of the production units).
  • Habitat: Oil-contaminated wastewater.
  • Isolator(s): Zayadan B.K., Dauletova A.A., B.K. Zayadan and A.A. Dauletova, K.Bolatkhan at the Biotechnology Laboratory of Al-Farabi Kazakh National University
  • Year of entry into the collection: 2024
  • Method of obtention: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
  • Genetic features: Wild type (WT)
  • Culture purity: axenic.

Morphology and Structure

The cells are solitary, with a regular elongated-oval, elliptical, or rod-like shape and strictly rounded ends, exhibiting a rich emerald-green color. The cell wall is smooth and thin, lacking spines or long needles; the cells are surrounded by a minimal, visually faint layer of exopolysaccharide slime. Reproduction occurs exclusively by binary transverse cell division in a single plane. According to Dussart’s size classification of plankton, based on the average metric characteristics of the cells (length: 1.5–3.5 μm, width: 1.0–2.0 μm), the strain belongs to nanoplankton (a group of medium-sized microorganisms ranging from 2 to 20 μm), bordering on the picoplankton fraction.

Cultivation

  • culture medium: BG-11 medium
  • temperature: 24–28 °C
  • light intensity: 60–120 µmol photons/m²/s
  • photoperiod: 16 h light / 8 h darkness
  • pH of the medium: 7.5–8.2

Physiological Characteristics

Halotolerant.

Long-term Preservation Conditions

maintained for long-term storage on BG-11 medium (liquid or solidified with 1.5% agar) at 12–15 °C under low light intensity (5–10 μmol photons m⁻² s⁻¹) with a 12 h light/12 h dark photoperiod. Storage on culture shelves with subculturing every three months is considered optimal. Storage in a refrigerator at 4–8 °C is also acceptable, provided that the cultures are not kept in complete darkness.

Genetic Information

GenBank accession №PZ747266

Field of Application

in fundamental research and biotechnology.