Nostoc calcicola CZS 2205

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan.
• Place of isolation: Almaty Region, Tuzkol lake.
• Habitat: hypersaline water body.
• Isolator(s): Sandybayeva S.K., Zayadan B.K., Akmukhanova N.R. at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of entry into the collection: 2022.
• Last identification: Sandybayeva S.K., Zayadan B.K., Akmukhanova N.R., 2023.
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type (WT)
• Culture state: Axenic culture.

Morphology and Structure

Cell size: predominantly 2–5 µm; trichome diameter 3.5–4.0 µm; heterocysts 5–6 µm; akinetes (spores) 7–8 µm. Cell organization: filamentous, colonial cyanobacterium; trichomes are tightly coiled and densely intertwined, forming filamentous clumps (aggregates) in liquid culture. Flagella: absent.

Cultivation

• Nutrient medium: liquid BG-11 medium without nitrogen (CN=0)
• Temperature: 28 ± 2 °C
• Light intensity: 53 µmol photons/m²/s
• Photoperiod: 16:8 h (light/dark)
• pH: 7.5
• Humidity: approximately 60%.

Physiological Characteristics

mesophile

Long-term Preservation Conditions

maintained in liquid nitrogen-free BG-11 medium at room temperature and subcultured every 2–3 months. Lyophilized biomass is stored at +4 °C under airtight conditions, protected from moisture, light, and oxygen, with a storage life of 1–3 years.

Deposition Information

Deposited in 2023 in the "Republican Collection of Microorganisms" of the Republic of Kazakhstan. Collection number: RKM 1061.

Field of Application

Fundamental research and biotechnology. The strain is a promising source of the bioactive secondary metabolite Nostocyclamide, which has potential application as a natural algicide against microalgae.

Bibliography

1. Akmukhanova, N. R., Seiilbek, S. N., Sandybayeva, S. K., Zayadan, B. K., Albay, M., & Kirbayeva, D. K. (2024). Allelopathic activity of cyanobacteria isolated from Lake Tuzkol. Journal of Phycology, 60, 1557–1566. https://doi.org/10.1111/jpy.13521

Patents and Other Intellectual Property

Utility Model Patent No. 9403: "The strain Nostoc calcicola CZS 2205 – a producer of biologically active substances with algicidal properties." Application No. 2024/0250.2 of February 21, 2024; published July 26, 2024, Bulletin No. 30. Inventors: Seiilbek Sandugash Nurlankyzy, Akmukhanova Nurziya Rakhmedievna, Zayadan Bolatkhan Kazykhanuly, Sandybayeva Sandugash Kalzhankyzy. Patent holder: Non-profit Joint Stock Company (NJSC) Al-Farabi Kazakh National University.