Chlamydomonas reinhardtii CC-124Res-1

History of Receipt and Isolation

• Country of origin: USA (original wild-type strain from the Chlamydomonas Resource Center, Duke University); Kazakhstan (obtainment of the mutant strain)
• Place of isolation: Biotechnology Laboratory of Al-Farabi Kazakh National University.
• Last identification: Zayadan B.K., Akmukhanova N.R., Sadvakasova A.K., 2013
• Method of obtainment: induced mutagenesis of the collection strain Chlamydomonas reinhardtii CC-124 and selection (mutagen — UV light, selective factor — heavy metal (cadmium))
• Genetic features: mutant strain
• Strain condition: axenic

Morphology and Structure

cells spherical, rarely ellipsoidal, 7.5–12.4 μm in diameter; cell wall thin, tightly adjacent to the protoplast, does not form a papilla at the anterior end; flagella length ½ times exceeds the cell diameter; chromatophore cup-shaped, smooth; one pyrenoid, in the strongly thickened posterior part of the chromatophore; eyespot hemispherical, in the anterior third of the cell; contractile vacuole 1, anterior; nucleus above the pyrenoid; sexual process isogamous; gametes ellipsoidal, formed 4–8 per cell, 8–12 μm in diameter each; zygotes spherical, with smooth wall, 16–18 μm in diameter

Cultivation

• Nutrient Medium: TAP medium (liquid and agarized)
• Temperature: 24–28 °C
• Light Intensity: 50–100 µmol photons/m²/s
• Photoperiod: 16:8 h (light/dark)
• pH: 7.0–7.5

Physiological Characteristics

resistant to cadmium ions at a concentration of 0.1 mg/mL; under optimal cultivation conditions, on days 7–8, the content of substances per 1.0–2.0 g/L of dry biomass is: proteins — 38–45 %, carbohydrates — 40–45 %

Long-term Preservation Conditions

the strain is maintained on liquid and 1.5–2.0% agar-solidified TAP medium. The storage temperature is 24–28 °C, light intensity is 10–30 µmol photons/m²/s, with a 12:12 h light/dark photoperiod. The strain is subcultured every 30–60 days.

Deposition Information

deposited in 2005 in the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration number - A-RKM 0175

Field of Application

fundamental and applied research in physiology, genetics, biotesting, bioremediation

Bibliography

1. Садвакасова, А. К. Получение мутантных штаммов Chlamydomonas reinhardtii устойчивых к кадмию, для применения в экобиотехнологии / науч. руководители Жубанова А.А., Заядан Б.К. – Астана, 2006.
2. Берсагурова А.М., Садвакасова А.К., Акмуханова Н.Р., Заядан Б.К. Скрининг диких и мутантных штаммов Chlamydomonas reinhardtii по чувствительности к кадмию // Тезисы докладов I Междунар. конгресса студентов и молодых ученых. – 2007. – С. 14.
3. Бауенова, М. Ө., Салех, М., Садвакасова, А. К., & Еркеш, Ж. (2014). Кадмийге сезімтал Chlamydomonas reinhardtii-дің мутантты штамдарын алу. Вестник КазНУ. Серия биологическая, 60(2), 182–186.

Patents and Other Intellectual Property

Invention Patent No. 29255. Application No. 2013/0367.1 dated March 26, 2013. Publication date: December 15, 2014, Bulletin No. 12. Title: "Method for the biological purification of domestic and industrial wastewater from organo-mineral impurities and heavy metal ions." Patent authors: Sadvakasova Asemgul Kalyikumarovna; Zayadan Bolatkhan Kazykhanovich; Kirbayeva Dariga Kenzhebaevna; Akmukhanova Nurziya Rakhmedievna; Beisenova Aizhan Zhumagazievna.