Chlorella vulgaris Z-1

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: sewage treatment facilities of Almaty city
• Habitat: wastewater
• Isolator(s): Zayadan B.K., Akmukhanova N.R. in the biotechnology laboratory of Al-Farabi KazNU
• Year of accession to the collection: 2002
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type
• Strain condition: axenic

Morphology and Structure

Cells are spherical, 1.7–3.3 µm in diameter; during autospore formation they reach 5.5 µm, and cells with vacuoles up to 6.0 µm. The cell wall is very thin. The chromatophore is broadly girdle-shaped, open or cup-shaped, with a pyrenoid; starch and oil droplets are observed. Nuclei are not visible. Reproduction occurs by autospores (4, rarely 2 or 8).

Cultivation

• Culture medium: Tamiya mineral medium (liquid and agarized)
• Temperature: 24–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH of the medium: 6.5

Physiological Characteristics

neutrophilic, mesophilic, autotrophic.

Long-term Preservation Conditions

Stored on liquid and 1.5–2.0 % agarized Tamiya mineral medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

Deposited in 2009 in the Republican Collection of Microorganisms of the Republic of Kazakhstan.

Field of Application

In fundamental research and biotechnology.

Bibliography

1. Заядан, Б. К., & Акмуханова, Н. Р. (2002). Микробалдыр Chlorella vulgaris Z-1 табиғи түрінің биомассасына жасанды экожүйедегі кейбір экологиялық факторлардың әсері. Вестник КазГУ. Серия экологическая, 3(15), 58–62.
2. Заядан, Б. К., Акмуханова, Н. Р., & Садвакасова, А. К. (2007). Биоаккумуляция ионов тяжелых металлов клетками микроводоросли Chlorella vulgaris Z-1. Вестник КазНУ. Серия биологическая, 1(31), 100–105.
3. Акмуханова, Н. Р., Заядан, Б. К., Садвакасова, А. К., Кирбаева, Д. К., Болатхан, К., & Бауенова, М. Ө. (2015). Микробалдыр Chlorella vulgaris Z-1 штамын ангидробиоз жағдайында сақтау нәтижелері. Вестник КазНУ. Серия биологическая, 64(2/2), 240–245.

Patents and Other Intellectual Property

Innovation Patent of the Republic of Kazakhstan No. 24332. Application No. 2010/0877.1 dated June 30, 2010. Publication date: October 15, 2013, Bulletin No. 10. Title: “Feed additive for increasing egg production in laying hens and a method for increasing egg production based on mixed cultures of microalgae.” Authors: Bolatkhan K. Zayadan; Dariga K. Kirbayeva; Azhar A. Zhubanova. Patent holder: Republican State Enterprise on the Right of Economic Management “Al-Farabi Kazakh National University”