Chlamydomonas reinhardtii CC CBn

History of Receipt and Isolation

• Country of origin: Russia (parental wild strain from the Peterhof collection of microorganisms, St. Petersburg); Kazakhstan (obtainment of the mutant strain)
• Place of isolation: at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Isolator(s): Zayadan B.K.
• Year of accession to the collection: 2001
• Last identification: Zayadan B.K., Akmukhanova N.R., Sadvakasova A.K., 2013
• Method of obtainment: mutagenesis (UV-induced + selection for antibiotic resistance).
• Genetic features: pigment mutant strain
• Strain condition: axenic

Morphology and Structure

Unicellular; the cell is enclosed in a cytoplasmic membrane, to which a cell wall is adjacent from the outside, surrounded externally by a protective capsule. The cell contains one large chloroplast. The organism swims with the help of two anterior flagella and exhibits phototaxis, which is perceived by a specialized eyespot (stigma).

Cultivation

• Nutrient Medium: TAP medium (liquid and agarized)
• Temperature: 24–28 °C
• Light Intensity: 50–100 µmol photons/m²/s
• Photoperiod: 16:8 h (light/dark)
• pH: 7.0–7.5

Physiological Characteristics

mesophile, autotroph, has a photosynthetic defect without chlorophyll b.

Long-term Preservation Conditions

maintained on liquid and 1.5–2.0% agarized TAP medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

deposited in 2003 into the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration Number: A-RKM 0128

Field of Application

in fundamental research and biotechnology.

Bibliography

1. Чунаев, А. С., Столбова, А. В., Никулина, К., & Заядан, Б. К. (1999). Генетика биосинтеза фотосинтетических пигментов Chlamydomonas reinhardtii. Вестник Санкт-Петербургского университета. Серия 3. Биология, 4(24), 122–131.
2. Sadvakasova, A. K., Akmukhanova, N. R., Matorin, D. N., Protopopov, F. F., Alekseev, A. A., & Zayadan, B. K. (2016). Pigment mutants of the green microalga Chlamydomonas reinhardtii: Morphological properties and photosynthetic performance. Russian Journal of Plant Physiology, 63(4), 443–450. https://doi.org/10.1134/S1021443716040130