Chlorella sp. 3

History of Receipt and Isolation

• Country of origin: Russia (parental strain from the IPPAS collection, Moscow); Kazakhstan (storage location)
• Place of isolation: Collection of Microalgae Cultures of the Institute of Plant Physiology, Moscow (IPPAS)
• Last determination: Zayadan B.K., Akmukhanova N.R. in the Laboratory of Biotechnology of al-Farabi Kazakh National University, 2015
• Year of accession to the collection: 2001
• Method of obtainment: collection strain
• Genetic features: Wild type
• Strain condition: axenic

Morphology and Structure

Spherical cells with a thin outer membrane are surrounded by a thick layer of mucus. The diameter of the cells, excluding the mucous layer, ranges from 1.8 to 6.5 micrometers (with the minimum size of young cells being 1.8–3.8 micrometers), while the total diameter of the cell, including the mucous sheath, reaches 23.0 micrometers. No pyrenoid is found in the internal structure; it is characterized by the presence of a cup-shaped chromatophore. A clearly differentiated nucleus is not visualized in living cells.

Cultivation

• Culture medium: Tamiya mineral medium (liquid and agarized)
• Temperature: 24–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH of the medium: 6.0–7.0

Physiological Characteristics

Mesophile, autotroph, neutrophile.

Long-term Preservation Conditions

Stored on liquid and 1.5–2.0 % agarized Tamiya mineral medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

Deposited in 2003 in the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration number - A-RKM 0121.

Field of Application

In fundamental research and biotechnology.

Bibliography

1. Буянхишиг, Н., & Заядан, Б. К. (1989). Результаты определения автоспора и длительности клеточного цикла культуры одноклеточных зеленых водорослей Chlorella sp. Научные труды Института общей и экспериментальной биологии, 24, 24–29.
2. Zayadan, B. K. (1991). Passportization of mutant strains of Chlorella sp. /Catalog of Microalgae Cultures in USSR Collections (p. 27). Moscow.
3. Заядан, Б. К. (2001). Жасыл балдырлар Chlorella клеткаларының ауыр металдарға төзімділігі мен олардың биомассасындағы цистеин мөлшері арасындағы қатынасты зерттеу нәтижелері. Вестник КазГУ. Серия биологическая, (2), 117–121.

Patents and Other Intellectual Property

Patent No. 29255. Application No. 2013/0367.1 dated 26.03.2013. Publication date: 15.12.2014, bulletin No. 12. Title: "Method of biological treatment of domestic and industrial wastewater from organo-mineral impurities and heavy metal ions." Patent authors: Sadvakasova Asemgul Kalyikumarovna; Zayadan Bolatkhan Kazykhanovich; Kirbayeva Dariga Kenzhebaevna; Akmukhanova Nurziya Rakhmedievna; Beisenova Aizhan Zhumagazievna.