Chlorella pyrenoidosa C-2

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: Almaty city, sewage treatment facilities
• Habitat: wastewater
• Isolator(s): Zayadan B.K., Akmukhanova N.R. in the biotechnology laboratory of Al-Farabi KazNU
• Year of accession to the collection: 2001
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type (WT)
• Strain condition: axenic

Morphology and Structure

Cells are spherical, 3.3–7.8 µm in diameter (reaching up to 10 µm during autospore formation). The cell wall is thin; the chromatophore is flat, girdle-like (with non-closed ends), or cup-shaped; and contains pyrenoids surrounded by a starch sheath consisting of 2–4 segments. The nucleus is sometimes visible. Reproduction occurs by autospores (4–8, rarely 2 or 16).

Cultivation

• Culture medium: Gromov mineral medium No. 6 (liquid and agarized)
• Temperature: 24–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH of the medium: 7.5

Physiological Characteristics

Mesophile, autotroph. High growth rate. Protein synthesis, β-carotene, amino acids, and vitamins are observed.

Long-term Preservation Conditions

Stored on liquid and 1.5–2.0 % agarized Tamiya mineral medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

Deposited in 2003 in the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration number - A-RKM 0117.

Field of Application

In fundamental research and biotechnology.

Bibliography

1. Буянхишиг, Н., & Заядан, Б. К. (1989). Результаты определения автоспора и длительности клеточного цикла культуры одноклеточных зеленых водорослей Chlorella sp. Научные труды Института общей и экспериментальной биологии, 24, 24–29.
2. Zayadan, B. K. (1991). Passportization of mutant strains of Chlorella sp. /Catalog of Microalgae Cultures in USSR Collections (p. 27). Moscow.
3. Заядан, Б. К. (2001). Жасыл балдырлар Chlorella клеткаларының ауыр металдарға төзімділігі мен олардың биомассасындағы цистеин мөлшері арасындағы қатынасты зерттеу нәтижелері. Вестник КазГУ. Серия биологическая, (2), 117–121.